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Fig. 6. Shh/Shh in limb buds. (A) PCR analysis of forelimbs, hindlimbs, heads and bodies from E11.5 embryos of wild-type/wild-type, wild-type/raz and raz/raz genotypes. Note the low, but definitive band in lanes with raz/raz limb bud samples (lanes 2, 3 and 10). (B) Band quantitation by image analysis of the gel in A. (C) Western blot of E11.5 forelimbs and hindlimbs from wild-type/wild-type, wild-type/raz and raz/raz embryos. (D) Image analysis of bands in C based on tubulin content of each sample.