Fig. 2. MCSP expression by cultured human epidermal keratinocytes. (A-C) Abortive (B) or actively growing (A,C) clones of primary human keratinocytes double labelled for MCSP and ß1 integrins. (D) Double labelling of MCSP and actin in a single keratinocyte, demonstrating that MCSP does not localize with the termini of actin filaments and so is not a component of focal adhesions. The MCSP and actin labelling are shown separately (top) and merged (bottom). (E) Immunofluorescence labelling of SCC4 squamous cell carcinoma keratinocytes showing the expression of MCSP at cell-cell borders and on apical microvilli. Scale bars: 10 µm (D) and 20 µm (E).