Fig. 3. Comparison of surface levels of MCSP and ß1 integrins in primary human keratinocytes by flow cytometry. Profiles of basal keratinocytes are shown following gating out of differentiated cells. (A) Dot plot of total basal keratinocyte population. Vertical and horizontal lines represent the fluorescence of single antibody controls. Boxes represent cells selected on the basis of highest or lowest 20% of expression of ß1 integrins (FL-1) or MCSP (FL-3). (B) MCSP fluorescence of total basal keratinocytes (Total) or 20% of cells with highest ß1-integrin expression (ß1-High) or 20% of cells with lowest ß1-integrin expression (ß1-Low). (C) ß1-Integrin fluorescence of total basal keratinocytes (Total) or 20% of cells with highest MCSP expression (MCSP-High) or 20% of cells with lowest MCSP expression (MCSP-Low). (B,C) Dotted lines show the fluorescence of cells labelled with secondary antibody alone.