Fig. 5. Expression of CD8/MCSP in primary human keratinocytes. (A) Amino acid sequence alignment of the cytoplasmic domain of human MCSP with the mouse and rat forms of the proteoglycan (AN2 and NG2, respectively). Boxes indicate amino acids completely conserved between all three species. Amino acid numbering is indicated for the human sequence. (B) Diagrammatic representation of CD8/MCSP and CD8 constructs. TM, transmembrane. (C,D) Flow cytometry of single cell suspensions of primary human keratinocytes (C) or SCC4 (D) transduced with empty vector, CD8 or CD8/MCSP and labelled with anti-CD8 (solid lines in C), anti-MCSP (black lines in D) or secondary antibody alone (dotted lines in C; grey lines in D).