Fig. 6. Lack of any effect of CD8/MCSP on terminal differentiation or clonal growth of primary human keratinocytes. (A) Flow cytometry of single cell suspensions of primary human keratinocytes expressing empty retroviral vector, CD8 or CD8/MCSP labelled with anti-involucrin antibody. The proportion of involucrin-positive cells (indicated by the M1 marker) is indicated. (B) Keratinocytes were plated in triplicate at clonal density onto mitotically inactivated fibroblast feeders and cultured for 10-14 days. The proportion of plated cells that formed clones of any size (%CFE) and the proportion of clones that were abortive, consisting of terminally differentiated cells (%Ab), were determined.