Fig. 2. abi3, fus3 and lec2 mutations affect At2S3 expression in seeds. (A) Northern blot analysis of At2S3 and At2S3::GFP expression. All plants analysed are homozygous for the At2S3::GFP transgene introduced in wild-type Col-0 (lanes 1-3), abi3 (lanes 4-6), lec2 (lanes 7-9) and fus3 (lanes 10-12). Total RNA was extracted from 11 DAP siliques (lanes 1, 4, 7, 10), 12 DAP siliques (lanes 2, 5, 8, 11) and 13 DAP siliques (lanes 3, 6, 9, 12). (B) The At2S3 expression pattern is altered in abi3, fus3 and lec2 mutants. Non-radioactive in situ hybridisation with an At2S3 probe was performed on 11-13 DAP seeds of the same genotypes as in A: Col-0 (panels 1 and 7), fus3 (panels 2, 3, 8), abi3 (panels 4 and 9), and lec2 (panels 5, 6, 10) homozygous for the At2S3::GFP transgene. In wild type, expression was detected in both cotyledons (c), the embryo axis (a) and endosperm cell layer (e). Arrows in panels 5 and 6 indicate sectors of lec2 cotyledons with reduced At2S3 expression. Scale bars: 50 µm (1 to 6) and 25 µm (7-10).