Fig. 4. Storage protein gene expression is dramatically reduced in lec2 fus3 double mutant. (A,B) At2S3::GFP seeds in Col-0 background (top) and lec2 fus3 At2S3::GFP seeds (bottom) at 16 DAP visualised under white light (A) or blue light (B). (C-E) Green fluorescence in lec2 fus3 At2S3::GFP (D) embryos as compared to At2S3::GFP Col-0 (C) at 14-16 DAP. (E) Same embryo as in D but under white light to show the presence of green and purple pigments in cotyledons. (F) Quantification of storage protein gene expression by northern blot analysis of seed RNA from wild-type, abi3, fus3, lec2 and lec2 fus3 seeds in the At2S3::GFP background. Specific probes used were At2S1 (lanes 1), At2S2 (lanes 2), At2S3 (lanes 3), At2S4 (lanes 4), CRUCIFERIN A (lanes a), CRUCIFERIN B (lanes b), CRUCIFERIN C (lanes c). (G) Analysis of ABI3 expression by western blot of total protein extracts from wild-type, abi3, fus3, lec2 and lec2 fus3 seeds.