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Fig. 5. FUS3 and LEC2 promoter activities in embryos and endosperm. LEC2::GUS (A-F) and FUS3::GUS (G-K) activities were assayed in whole seeds (A,B,H,I: globular stages), isolated embryos (C,D, torpedo stages; E, early mature embryos around 12 DAP; J, heart stage; K, mature embryo before desiccation) or sections of seed envelopes (F,G). FUS3::GUS (M,O,Q) expression pattern and At2S3::GFP green fluorescence (L,N,P) were compared in individual lec2 mutant embryos just before desiccation (at 15-16 DAP). Scale bars: 50 µm. (R) Time course of FUS3 and LEC2 mRNA expression during silique development analysed by RT-PCR. The developmental stage of samples is indicated in DAP. EF1{alpha} was used as a control.