Fig. 2. Expression pattern of Cct1 in wild-type ovaries. (A) X-gal staining showing BN81 enhancer trap expression in the anterior follicle cells from stage 7 to stage 10. At stage 10, expression is seen in the border cells, nurse cell associated follicle cells and centripetally migrating follicle cells. (B-D) Cct1 expression detected by whole-mount in situ hybridization. (B) Cct1 is first expressed in the follicle cells in region 2B of the germarium. (C) During mid-oogenesis, Cct1 expression is detected in the anterior follicle cells. Expression begins during stage 7 in the follicle cells at the anterior-most end of the egg chamber. At stage 10B, Cct1 expression is seen in the nurse cell associated and centripetally migrating follicle cells. (D) From stages 12-13, Cct1 is expressed in the dorsoanterior follicle cells and the posterior follicle cells. Cct1 expression can also be detected in the germline cells at various levels throughout oogenesis.