Fig. 3. Defect in germline stem cell maintenance in Cct1 mutants. (A) Wild-type ovariole labeled with an antibody to Vasa protein, which marks the germline cells (green) and phalloidin to visualize the actin cytoskeleton (red). Ovaries from Cct1⁹⁹ mutants contain ovarioles with only one or two egg chambers. (B,C) Germaria stained with antibodies to Vasa (green) and -Spectrin (red), which marks the spectrosomes and fusomes. (B) Wild-type germaria contain two or three germline cells, marked by the presence of spectrosomes, and seven or eight dividing germline cysts with fusomes. Arrowhead indicates a spectrosome. (C) Cct1⁹⁹ mutant germaria sometimes contain few or no germline cells with spectrosomes. Scale bars: 20 µm.