Fig. 6. Branched ovariole and shortened ovariolar sheath in Cct1 and Drosophila Wnt4 mutant ovaries. Ovaries were stained with Orb antibody (red) to label the oocyte, phalloidin (green) to visualize the actin cytoskeleton and Hoechst (blue) to label the DNA. (A) Cct1⁹⁹ mutant ovary with branched ovarioles. (B) Branched ovarioles in Drosophila Wnt4^C1 mutant ovary. (C) Wild-type ovariole showing egg chambers stretched out within ovariolar sheath. (D) Cct1⁹⁹ mutant ovariole with egg chambers curled up within the sheath.