Fig. 8. Shortened operculum phenotype and regulation of expression by dpp signaling. (A,B) Chorion phenotypes. (A) Wild-type egg. (B) Egg laid by Cct1¹²⁴/Cct1²⁹⁹ mutant. Females hypomorphic for mutations in Cct1 lay eggs with shortened opercula. (C,D) In situ hybridizations showing Cct1 expression. (C) Cct1 expression in anterior follicle cells in a wild-type egg chamber. (D) Cct1 expression in a dpp misexpression background. When dpp is ectopically expressed in all of the follicle cells, Cct1 is also expressed in all the follicle cells. (Uneven staining caused by patchy expression of Gal4 driver.) (E) Expression of the enhancer trap BN81 (green) in a follicle cell clone mutant for Mad¹², which is marked by the loss of Myc expression (red). (F) Expression of phosphorylated-SMAD (green) in a Cct1⁹⁹ mutant follicle cell clone, indicated by lack of Myc expression (red).