Fig. 3. The cmlc2-driven EGFP is expressed in cardiomyocytes. (A) The wild-type primitive heart is a long tubular structure. (B) The primitive heart tube is short in the had mutant embryo, but has a stronger EGFP signal. Arrows point to the heart. (C) A typical image of dissociated cardiomyocytes from a cmlc2:EGFP embryo at 24 hpf. (D) Comparison of the numbers of cardiomyocytes in wild-type and had mutant embryos at 24 hpf. The y-axis shows the number of EGFP-positive cells.