Fig. 3. Retarded GT outgrowth associated with an increase of apoptosis. (A-F) GTs at 12.0 dpc were cultured for 3 days (A,B) or for 24 hours (C-F). Apoptotic cells were increased in the GT epithelium and its adjacent mesenchyme after the staurosporine treatment, which was associated with retarded GT outgrowth proximodistally. (G,H) GT explants between 11.5 dpc and 12.0 dpc treated with anti-SHH antibody (5E1) showed a reduction in Fgf8 expression after 24 hours of culture. (I,J) An increase of apoptosis was observed by the 5E1 treatment. The number of Nile Blue-positive cells was significantly increased by 5E1 treatment. (A-D,G,H) Ventral views; (E,F,I,J) frontal views. Scale bar: 250 µm.