Fig. 2. Embryonic phenotypes associated with ed mutations. Ventral views of wild-type (WT) (A) and ed^2B8/ed^2B8 (B) embryos stained with anti-HRP antibody. ed^2B8 homozygous embryos exhibit a hyperplasia of the CNS. This hyperplasia is accompanied by loss of epidermal structures. (C-F) Lateral views of the embryos. Cuticle preparations of WT (C) and ed^2B8/ed^2B8 (D) embryos. Asterisks in C mark denticle belts, characteristic structures of the ventral cuticle. ed^2B8 homozygous embryos show an extensive loss of ventral and procephalic cuticle. (E,F) Lateral views of WT (E) and ed^2B8 homozygous (F) embryos labeled with anti-Deadpan antibody. Arrowheads in F mark the ectopic neuroblasts present in ed mutant embryos.