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Fig. 3. Ectopic expression of FGFs and formation of ectopic otic vesicles. (A) Construct for the generation of transgenic mice. An Epha4 r3/r5 enhancer in combination with a ß-globin minimal promoter is used to drive expression of FGFs and an IRES-lacZ reporter gene in the hindbrain. (B) Formation of an ectopic vesicle (arrow) anterior to the otic vesicle (OV) in an FGF10 transgenic embryo. (C) Section through the hindbrain of an FGF10 transgenic embryo stained for lacZ. An ectopic vesicle (arrow) has formed next to rhombomere (r) 4. (D,E) Expression of Dlx5 and Lmx1 in ectopic vesicles (arrows) of FGF10 transgenic mice. Scale bars: in C, 80 µm for C and 200 µm for B,D; in E, 200 µm for E.