Fig. 2. Cyclin A promotes the encore extra division phenotype. (A-C) In wild-type germaria Cyclin A (red) associates with the fusome (green stained with phalloidin for actin) in dividing cystoblasts (arrow and inset). (D-F) Association of Cyclin A with the fusome in encore mutant germaria persists in posterior regions of the germarium (arrow and inset). (G) encore mutant females raised at 25°C produce 55% of the egg chambers with 32 cells. Reducing Cyclin A gene dose has no effect on the encore cell division phenotype. However overexpression of the HS-rux transgene in an encore mutant background reduces the 32 cells egg chamber phenotype from 55 to 25%. Additional reduction of Cyclin A gene dose results in 20% of the egg chambers containing 32 cells. (H) Western blot showing that the amount of Cyclin A protein in encore mutant extract is increased compared with the wild-type extract. Equivalent amounts of germaria-enriched extract were loaded in both lines as seen with the protein dye ponceau (pink).