Fig. 1. Expression of L in the larval eye disc is initiated earlier than pnr and Iro-C genes. All eye discs in this and subsequent figures are oriented anterior towards the right and dorsal towards the top. Eye disc of first- (A,A', arrows), early second- (B,B', arrow), and late second- (C,C') instar larvae stained for pnr (green), L (red) and Elav (blue). Expression of pnr (green, arrow) begins in late first- to early second-instar disc (B,B') in a small group of cells at the dorsal margin. Expression of L (red) and mirr (green) in first- (D,D', arrowhead), early second- (E,E') and mid-second- (F,F') instar eye disc, respectively. mirr is expressed in a broader domain in the dorsal eye as compared with the pnr. Individual channels of the images are also shown ('). Magnifications of the images are same.