Fig. 2. Pnr is not essential for DV patterning of eye during embryogeneis. Ush was misexpressed in eye by ey-GAL4; UAS-Ush (ey>Ush) and cultures were shifted to 29°C during different stages of development. (A) Three different restrictive temperature conditions used. (B,D-F) Eye discs were stained for Wg or L (blue), Elav (red) and Ey (green). (B-D) Cultures maintained at 29°C throughout development served as controls and resulted in elimination of entire eye field (B) in eye disc and (C) in adult eye. (D) Some weaker phenotypes of very small eye marked by Elav-positive cells were also observed. (E) Maintenance at 18°C during embryonic development and then shift to 29°C for subsequent development also resulted in complete elimination of eye field. (F,G) When cultures were shifted to 29°C during embryonic development to block pnr activity and later allowed to develop at 18°C, they did not show any eye suppression phenotype (F) in the eye disc and (G) in adult eye.