Fig. 5. Loss-of-function of pnr and Iro-C changes dorsal eye sensitivity to ventral. (A,B) Overexpression of L by ey>L causes selective ventral eye suppression in disc (A, arrow) and adult (B, arrow). Loss-of-function clones of pnr were generated in eye using EGUF approach, which resulted in dorsal eye enlargement (C) in disc and (D) in adult. In the ventralized disc with EGUF pnr clones in dorsal when L (ey>L) was overexpressed resulted in small eye because of suppression of eye fate on both dorsal and ventral margin of (E) disc (arrows) and in (F) adult eye (arrowhead). Misexpression of Ser^DN (ey> Ser^DN) in the ventralized disc with EGUF pnr clones completely abolished the entire eye fate (G) in disc and in (H) adult eye. Loss-of-function clones of Iro-C show dorsal eye enlargement in (I) disc. (J) EGUF Iro-C clones in eye disc also result in dorsal eye enlargements. (K,L) In the ventralized eye disc mutant for Iro-C, overexpression of ey>L (K) or ey>Ser^DN (L) results in suppression of eye on both DV margins and complete removal of the eye fate, respectively.