Fig. 10. Formation of NLBs from miRet51 homozygous mice.
Postnatal gut was isolated from individual P2-8 mice born to
miRet51 heterozygous parents, dissociated and cultured as
described. (A,B) NLBs containing large numbers of TuJ1+ and GFAP+ cells formed
in gut cultures from miRet51 animals. (C) In addition,
retroviral transduction of NLBs resulted in the efficient isolation of EPCs
which generated neuron- and glia-containing colonies (a sector of which is
shown here).