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Fig. 10. Formation of NLBs from miRet51 homozygous mice. Postnatal gut was isolated from individual P2-8 mice born to miRet51 heterozygous parents, dissociated and cultured as described. (A,B) NLBs containing large numbers of TuJ1+ and GFAP+ cells formed in gut cultures from miRet51 animals. (C) In addition, retroviral transduction of NLBs resulted in the efficient isolation of EPCs which generated neuron- and glia-containing colonies (a sector of which is shown here).