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Fig. 2. NLBs do not form in gut cultures from Retk- homozygous embryos. Cultures were established from individual guts dissected from E11.5 embryos from Ret+/k- heterozygous parents. Neuron- and glia-containing NLBs formed readily in cultures of wild-type (A,B) and Ret+/k- (not shown) embryos but not in cultures from Retk- homozygotes (C,D).