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Fig. 3. Influence of O-fucose site mutants on Notch activity. WG expression (magenta) in wing discs expressing a Notch receptor with O-fucose attachment sites mutated. Arrows point to ectopic expression. (A) ptc-Gal4 UAS-N-EGF24f[c]. (B) ptc-Gal4 UAS-NEGF26f[b]. (C) ptc-Gal4 UAS-N-EGF24+26f[a]. (D) ptc-Gal4 UASN-EGF31f[b]. (E) ptc-Gal4 UAS-N-EGF12f[M4a]. (F) ptc-Gal4 UAS-N-EGF12f[M4a]UAS-fng[22a]. Fringe also results in Notch activation in ventral cells.