Fig. 6. N-EGF12f can rescue neurogenesis in Notch embryos. Embryos from a cross of N^55e11/FM7; UAS-NEGF12f[M4a]/+ to da-Gal4, stained for expression of ELAV (green), Notch (red) and SXL (cyan). Absence of SXL identifies embryos as male. Anti-Notch staining allowed effective identification of embryos with Notch overexpression, but did not allow effective discrimination between zygotically wild-type and Notch mutant embryos. Staining with the markers thus allowed division of male embryos into four classes, as indicated in Table 1. (A) Class II embryo. (B) Class III embryo. A portion of a female embryo is also visible (asterisk).