Fig. 7. Mutation of the O-fucose site in EGF12 enhances Notch-ligand binding. Histograms show the results of binding assays conducted with conditioned media, including 4800 mOD/minute AP activity of the indicated fusion proteins (Media), mixed with (A) Stably transfected DL-expressing cells, (B) transiently transfected SER-expressing cells, or, as a control, transfected S2 cells. Fc:AP is a control protein, consisting of the Fc domain of human IgG fused to AP. Values represent an average of three experiments, error bars indicate one s.d.