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Fig. 2. Expression of Oct4 (GOF18-deltaPE)-GFP transgenes in gastrulating embryos. Fluorescence images of embryos at (A) E6.5 (early streak stage), (B) E6.75 (mid-streak stage), (C) E7.25 (early allantoic bud stage). (D,E,F) Extra-embryonic mesoderm of Oct4-GFP embryos at mid-allantoic bud stage showing expression of Oct4-GFP (D arrowheads) and stained with anti-PGC7 antibody (E). (F) Merged image. The cells that express intense Oct4-GFP (D, filled arrowheads) in base of allantoic bud also stained with anti-PGC7 antibody (E, open arrowheads). Scale bar: 200 µm (A-C); 25 µm (D-F).