Fig. 7. Specific expression of E-cadherin within the cluster of PGC precursors. Explants of Oct4-GFP extra-embryonic mesoderm at E6.75 were cultured for 9-10 hours, and stained with anti-PGC7 antibody (B) or with anti-E-cadherin antibody, ECCD-2 (E). Oct4-GFP expression (A,D). (C,F) Merged images with TOTO-3 stained nuclei. Cluster of GFP-expressing PGC precursors that are PGC7/stella negative exclusively maintained E-cadherin expression. Scale bar: 50 µm.