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Fig. 8. Extra-embryonic mesoderm develops normally in cultured explants with E-cadherin-blocking antibody. Explants of extra-embryonic mesoderm were cultured for 15 hours with ECCD-1 (A-D), or with ECCD-2 (E-H). Cultured explants were stained with anti-PGC7 (A,E) and anti-Flk1 antibodies to show differentiation of extra-embryonic mesoderm (B, F). (C,G) Merged and (D,H) phase contrast images. Scale bar: 100 µm.