Fig. 9. Inhibition of migrating PGC emergence by E-cadherin-blocking antibody in prolonged culture. Extra-embryonic mesoderm explants at E6.75 (A,B) and E7.25 (C,D) were cultured with ECCD-1 (A,C) or ECCD-2 (B,D) for 48-60 hours. Emergence of PGCs was detected by ALP staining. Scale bar: 200 µm.