Fig. 4. Overexpression of Echinoid affects Dl levels but not N. Single confocal sections of dpp-Gal4/UAS-ed wing discs. (A,B) Immunohistochemical detection of Ed (green) and Dl (red). (A) Apical cell surface. (A') Red channel (Dl). (B) Confocal section taken just below the apical cell surface. (B') Red channel (Dl). (C) Immunohistochemical detection of Ed (green) and N^ECD (red). (C') Red channel (N^ECD). The levels of Dl fluorescence are decreased both at the cell membrane and in intracellular vesicles while the levels of N are unaffected.