Fig. 2. Wg and Egfr signalling pathways are not required for the development of the PE. (A-C) Clones lacking Wg signalling because of E-Cadh^intra5 overexpression (green). These clones accumulate the Wg signalling transcriptional effector Arm (red) in the cytoplasms (A, 24-48 hours AEL). The arrowhead indicates the region of the clone magnified in the insets. (B) E-Cadh^intra5 overexpression reduces the proliferation and survival of wing-notum cells (arrowheads), but have no effect in peripodial cells (outlined clone). Wg is detected in red. Clones generated 24-48 hours AEL. (C) These clones also eliminate the expression of zfh-2 (red) in cubic cells (inset at higher magnification of the outlined clone). Clones generated 48-72 hours AEL. (D) Clones lacking Egfr signalling because of overexpression of DN-Raf^3.1 (green, 24-48 h AEL) reduce iro-C expression (red) in cubic cells (inset at higher magnification of the region pointed by the arrowhead). The same clone does not affect the proliferation and survival of squamous cells (arrow).