(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 4. hlh-14 mutants are missing HSN, PHB, and PVQ neurons. Images in this series show lateral views of either wild-type (A,C,E) or hlh-14 mutant (B,D,F) larvae expressing GFP reporters to visualize the HSN, PHB or PVQ neurons. (A,B) Anterior is towards the left and ventral is upwards. (A) GFP expression in an hlh-14(gm34)/mIn1; kyIs179[unc-86::gfp] larva. As this worm is only heterozygous for the hlh-14(gm34) mutation, it appears wild type and expresses GFP in the HSN neuron visible in this focal plane (arrow). The GFP expression in the pharynx of this worm indicates the presence of the mIn1 balancer, and hence, a wild-type copy of the hlh-14 gene. (B) GFP expression in an hlh-14(gm34); kyIs179 larva. No HSNs are visible by GFP expression. The arrow indicates the position where the HSNs would be in a wild-type animal. (C) GFP expression in the tail of a wild-type gmIs12[srb-6::gfp] larva. GFP is visible in both phasmid neurons, PHA and PHB (two arrows), sensory neurons in the tail. (D) GFP expression in an hlh-14; gmIs12 larva. Only one phasmid neuron (PHA) is visible; PHB is missing. (E) GFP expression in an hlh-14(gm34)/mIn1; kyIs39[sra-6::gfp] larva. As this worm is only heterozygous for the hlh-14(gm34) mutation, it appears wild type and expresses GFP in the PVQ neuron visible in this focal plane (arrow). The GFP expression slightly anterior to PVQ is due to the presence of mIn1. (F) GFP expression in an hlh-14(gm34); kyIs39 larva. No PVQs are visible by GFP expression. The arrow indicates the position of the PVQs in a wild-type animal.