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Fig. 1. Transfection of Mitf into ES-like cell and fibroblast cell lines of medaka. (A) Non transfected MES-1 cells. (B) Bright field view of A; (C) MES-1 cells 4 days after transfection with XmaMitf and GFP vectors (arrow shows a faintly pigmented cell). (D) Bright field view of C. (E) Terminally differentiated melanocyte seven days after the transfection of MES-2 cells. (F) Dark field showing GFP expression. (G) Melanocytes in MES-1 transfected cells 12 days after the transfection (arrows indicate melanocytes). (H) Pigmentation in OLF cells transfected with XmaMitf (arrow indicates pigmented cell). Scale bars: 100 µm.