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Fig. 4. Expression assays of MES-1 transfected cells. (A) Transfected with XmaMitf. (B) Transfected with the mutant XmaMitf. nt, non-transfected cells; -, no cDNA (negative control); C, positive control. cDNA from medaka skin probed for tyrosinase, Tyrp1, Dct, Kit, Fms, Aim1, Ednrb, Tbx2, P53, OlaMitf and Actin. cDNA from 5-day-old medaka embryos probed for Erbb3, Sox10, Ap2, Snail2, Ncam and enolase. cDNA from adult muscle for Myf5 and for XmaMitf, MES-1 cells transfected with the XmaMitf construct.