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Fig. 12. VCS expression patterns. (A) RT-PCR analysis of VCS and {alpha}-tubulin (control) expression in RNA isolated from roots, hypocotyls, cotyledon, apices (leaves plus meristem), mixed age siliques, and entire seedlings (Whole). Genomic controls show the amplified fragment size that includes intron sequences. (B-H) GUS staining from a pVCS::GUS transgene. (B) 6-day cotyledon; (C) first leaf from a 4-day plant; (D) young leaves from a 9-day plant; (E) first leaf from a 9-day plant. (F) leaf trichome; (G) leaf vein; (H) stomata in the leaf. Scale bars: (B-H) 100 µm.