Fig. 3. Leaf defects in internal tissues of vcs mutants. (A-F) Cross sections of the first leaf from 12-day Ler (A-C) and vcs (D-F), grown at (A,D) 29°C; (B,E) 22°C; (C,F) 16°C. (G-L) Dark-field images of cleared first leaf showing the patterns of veins in Ler (G,I,K) and vcs (H,J,L) grown for (G,H) 12 days at 22°C; (I,J) 11 days at 29°C; (K,L) 15 days at 16°C. (M,N) DIC images of the primary vein from leaves shown in G and H. (O,P) Cotyledon vein pattern of 12-day 22°C-grown Ler (O) and vcs (P) show similar vein patterns, although cotyledon shape differs slightly. (Q,R) DIC images of the primary vein of cotyledons of Ler (Q) and vcs (R), showing ectopic tracheary elements surrounding the vcs primary vein. Scale bars: (A-F) 50 µm; (G-L,O,P) 1 mm; (M,N,Q,R) 100 µm.