Fig. 5. Temperature shift experiments reveal a requirement for VCS throughout leaf development. (A) Seedling morphology of 14-day temperature-shifted vcs mutants. The top row shows plants germinated at 29°C and transferred to 16°C on the day indicated under each plant. The lower row shows plants germinated at 16°C, and transferred to 29°C. (B) Representative vein patterns from selected temperature shift time points. The red bars indicate the duration of time at 29°C, and whether this exposure was at the beginning or the end of the 14-day growth period. Scale bars: (A) 5 mm; (B) 200 µm.