Fig. 8. DR5 expression and auxin inducibility in vcs leaves. DR5 expression in developing leaves of wild type (A-C) grown at 22°C (wild-type DR5 expression was similar regardless of growth temperature). (D-F) vcs mutants show DR5 expression at the distal end of the developing leaf (D, 29°C-grown) and in procambium (E,F, 22°C-grown). DR5 auxin induction was compared for 8-hour incubation in water (G,I) or 5 µM 2,4-D (H,J) in the wild type (G,H) and vcs (I-J). Data shown is for 29°C-grown tissue; similar results were observed for plants grown at 22°C and 16°C. Scale bars: (A-F) 100 µm; (G-J) 1 mm.