Fig. 9. vcs mutants show heightened sensitivity to polar auxin transport inhibitor NPA regardless of growth temperature. (A-H) 29°C-grown 9-day plants, (I-P) 22°C-grown 12-day plants, and (Q-X) 16°C-grown 15-day plants. (A,I,Q) Ler controls and (B,J,R) Ler grown in medium containing 1 µM NPA. (C,K,S) vcs controls and (D,L,T) vcs grown in 1 µm NPA. Dark-field images of cleared first leaves of Ler and vcs grown for 9 days at 29°C (E-H), 12 days at 22°C (M-P) and 15 days at 16°C (U-X). Ler controls (E,M,U), Ler 1 µM NPA (F,N,V), vcs controls (G,O,W) and 1 µM NPA (H,P,X). Scale bars: 1 mm.