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Fig. 1. Bax is expressed in migratory and post-migratory PGCs. Lane 1, E10.5 PGCs (gfp+); lane 2, E12.5 PGCs (gfp+); lane 3, E12.5 PGC cDNA RT-; lane 4, E12.5 whole embryo cDNA (+ control); lane 5, E11.5 PGCs (gfp+); lane 6, E11.5 somatic tissue from the genital ridge (gfp-); lane 7, E11.5 somatic tissue RT-. PGCs were prepared by flow cytometry and the purity of the sort was confirmed by RT-PCR for germ cell markers (Kit, Stag3) and somatic markers (Steel, cystatin 3 and Sparc) (data not shown).