Fig. 2. The survival of PGCs in the midaxial region of the embryo in Bax^-/- embryos at E10.5. (A,B) PGCs at 0 (A) and 700 (B) minutes in a slice from an E10.5 Bax^+/+ embryo. a, aorta; m, mesonephric duct; mes, mesentery of the gut. PGCs in the mesentery and midaxial region near the aorta fragment and die during the culture period. (C-F) Slices from Bax^-/- embryos, showing that after 700 minutes of culture, germ cells distant from the genital ridges do not fragment and die. C and E show slices at 0 minutes; D and F show the same slices at 700 minutes. Scale bar: 50 µm.