Fig. 5. Forty-eight hour survival of PGCs isolated from E10.5 (A,B), and E12.5 (C) embryos, and cultured on MEFs. The data are shown as 48 hour survival of PGCs from each embryo of one litter (left hand side) and the means (±s.e.m.) for embryos of the same genotypes in the litter (right hand panels). There is a significant increase in 48 hour survival of PGCs that are Bax^-/- (KO), compared with Bax^+/+ (WT) and Bax^+/- (HET) PGCs at 48 hours. Survival is measured as the % of germ cells counted in each sample at T0. See text for details.