Fig. 6. Shows the effect of an anti-Kit antibody or an isotype matched control antibody (J1.2) on 48 hour survival of either E10.5 (A) or E12.5 (B) germ cells. Survival is shown first as % of the number of PGCs counted in each embryo sample from a single litter at T0, and second, as means (±s.e.m.) for all embryos of the same genotype in the litter. In the E10.5 sample (A), 15 µg/ml of control (blue bars) and anti-Kit (red bars) were used. In the E12.5 sample (B), two concentrations of antibody were used; 15 µg/ml and 50 µg/ml. The effects of each dose are shown separately on the charts. Antibody concentrations of 15 µg/ml are represented by blue (control antibody) and red (anti-Kit antibody) bars (as in A), while the effects of 50 µg/ml of control or anti-Kit are shown as yellow and green bars, respectively. The anti-Kit antibody causes a significant reduction in germ cell survival at 48 hours in both E10.5 and E12.5 Bax^+/+ and Bax^+/- embryos, but not in Bax^-/- embryos. In the E12.5 litter, there was only one Bax^+/+ embryo, so there is no s.e.m.