Fig. 6. An average of 3.9 single cells do not stain with BamC antibody. (A) Individual germaria were scored for the number of cells that contain a spherical spectrosome and do not stain with BamC antibodies. The graph illustrates the number of germaria (y axis) that contained between 1 and 6 of such cells (x axis). (B) Wild-type ovaries were stained with anti-Vasa (blue), with the mAb 1B1 antibody (green) and with anti-BamC (red). Four cells that possess a single spectrosome but do not stain for BamC can be observed at the anterior of the ovariole (arrowheads). A cyst, which does stain with BamC antibody, can be seen just posteriorly (arrow). Scale bar: 20 µm. (C) An illustration of the possible outcomes of mitotic recombination in a pre-cystoblast or a GSC (see arrows). Cells carrying 0 copies of the lacZ transgene are depicted in white, whereas those carrying 1 or 2 copies of the transgene are light or dark green, respectively. (D) A germarium representing a GSC mitotic recombination event. Arrowheads mark the GSC and its progeny lacking lacZ (only one daughter cell is in plane). An arrow marks the `twin spot', which strongly expresses lacZ.