Fig. 5. Involvement of VM-hh in VM-PS subdivision. (A,B) Wild type. (C-D) hh^ts. (E,F) 48Y-GAL4/UAS-ciNZn. Late stage 11 (E) and stage 13 (A-D,F) embryos stained as indicated. (A-D) Elimination of hh activity during mid stage 11 to stage 12 (6.37.8 AEL) did not perturb the initiation of CON and VM-hh (data not shown), but caused loss of their expression at stage 13 (C,D). Note that CON expression in VM-PS3-5 still persists, even in the complete absence of hh activity (D; data not shown). (E,F) VM-hh expression was virtually normally initiated (E), but could not persist when UAS-ciNZn was driven by 48Y-GAL4 (F).