Fig. 7. Disruption of retinotectal projection by Grg4. (A,B) Terminal arborizations in the control (A) and Grg4-misexpressing region (B). HRP was injected in the contralateral eye at E15, the tectum was fixed at E17, and fibers were visualized with anti-HRP antibody on the slice. (C) Grg4 misexpression was assessed by immunohistochemical staining with anti-gag antibody on the same slice as B. In the control tectum, retinal fibers arborize superficial to lamina g (A), but in the Grg4-misexpressing tectum, retinal axons pass deeper beyond lamina g (arrowheads in B). The rectangle on C indicates approximate area of B. (D,E,E') Immunostaining with anti-neurofilament (D,E) and DAPI staining (E') on the sagittal section of the control (D) and Grg4-misexpresing region (E) on E12 tecta.