Fig. 3. Analysis of the snf¹⁴⁸ mutant phenotype. (A) Wild-type ovarioles stained with the nuclear dye DAPI. (B) Homozygous snf¹⁴⁸ mutant ovaries stained with DAPI contain tumorous egg chambers that are filled with large numbers of undifferentiated cells. (C) Wild-type ovarioles stained with an antibody directed against SNF, illustrating that SNF localizes to the nucleus. (D) Homozygous snf¹⁴⁸ mutant ovaries stained with an antibody directed against SNF. The magnification of a single mutant egg chamber (insert) illustrates that this mutation does not alter the nuclear localization of SNF. (E) Diagram of the reporter construct that mimics Sxl splicing in all tissues. The arrows below the construct show the positions of the nested PCR primer sets used for RT-PCR. (F) To analyze the RNAs produced by the Sxl reporter construct, total RNA was isolated from either adults or isolated ovaries of the indicated genotype, and analyzed by semi-quantitative RT-PCR.