Fig. 5. Pax1 and Pax9 can transactivate Bapx1 promoter. (A) Scheme of the Bapx1 genomic structure and of the p5.3Bp-luc reporter plasmid. The boxes indicate exons of Bapx1 (top) or the luciferase (luc) reporter (bottom). The coding regions of Bapx1 are shown by the black boxes, together with the first ATG codon (position +277) and the stop codon (TGA) in exon 2. The p5.3Bp-luc plasmid contains 5.4-kb genomic sequences from the Bapx1 promoter region and a part of 5' UTR (-5285/+109) upstream of the luciferase reporter. (B) Promoter activities of the p5.3Bp-luc construct alone or co-transfected with the indicated amounts of expression plasmids for Pax1 (pPax1) or Pax9 (pPax9), or both. Numbers inside or above the bars indicate time fold induction with respect to the basal p5.3Bp-luc promoter activity. Lines in the bars indicate s.d.