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Fig. 7. Analysis of maternal Ldb1 expression. Lanes 1-3, RT-PCR analysis for Ldb1 mRNA. Lane 1, oocytes; lanes 2 and 3, E7.5 embryos. Ldb1 mRNA is detected in wild-type (lane 2), but not in Ldb1 null mutant embryos (lane 3). Lanes 4-6, expression of control mRNA. The control mRNA is absent in the egg (lane 4), but present in E7.5 wild-type (lane 5) and Ldb1 mutant (lane 6) embryos.