Fig. 3. Müller glia and all of the major neuronal classes except bipolar cells (see Fig. 4) are present in the Chx10, Kip1 double null retinas at P19. Each cell type in the double null retina was found to have normal gross morphology and to be located in the appropriate layers, in comparison with wild type. By contrast, although all of these cell types are present in Chx10-null retinas, they lack normal morphology and organization. (A-D) Recoverin staining of photoreceptors in the outer nuclear layer. (E-H) Calbindin staining of horizontal and amacrine cells in the INL, and displaced amacrine and ganglion cells in the GCL.(I-L) CRALBP staining of Müller glia, which span the retina vertically. (M-P) Calretinin staining of AII amacrine cells in the INL and displaced AII amacrines in the GCL. (Q-T) ChAT staining of starburst amacrine cells in the INL and displaced starburst amacrines in the GCL. Scale bar: 50 µm.